Selective Plane Illumination Microscopy on a chip
The invention consists of a millimeter-sized optofluidic device (Lab-on-chip) that integrates a sheet of light illumination (SPIM microscopy) and automatic scanning of the sample in a microfluidic channel.
The device, built using a femtosecond laser micro-fabrication technique, includes one or more cylindrical optofluidic lenses with an engineered profile to obtain an aberration-free illumination plane that crosses the microfluidic channel.
Biological samples are circulated through a microfluidic pump and observed with a traditional microscope. An alignment procedure by the user is not necessary, thus allowing a fast and automatic three-dimensional scan of the sample. A high-throughput high-frequency acquisition system (> 1 sample / s, demonstrated on intact cell spheroids, 250 µm in diameter by acquiring 100 frames per sample).
- Optical Microscopy;
- Compact and portable: can be used as add-on in commercial upright or inverted microscopes without the need of any motorized stage;
- Versatile fluidic design: can image samples at different spatial scales, from single cells to organoids or embryos;
- Versatile optical design: suitable for multiple sided illumination, multicolor excitation and double sided detection;
- High-throughput: high acquisition rate (> 1 sample/s demonstrated on intact 250 µm diameter cellular spheroids and 100 frames/sample);
- Modular: easy integration of multiple lab-on-chip functionalities.