Introduction

Method for the preparation of platelet lysate free from contaminants that can generate transmission of diseases by viral, microbial, fungal or parasitic agents, called inactivated lysate, obtained as an adjuvant for cultivation, growth expansion of stem cells, in particular mesenchymal. The cells can advantageously be used for therapy and reintroduced into subjects.

Technical features

Method for the preparation of platelet lysate free of contaminants, with respect to the known art, comprising the steps of: isolating the buffy-coat fraction from whole blood obtained from at least two subjects; exposing the isolated buffy-coat fraction to a photochemical agent and to UV rays under conditions such as to obtain an isolated buffy-coat fraction free of contaminants; subjecting said fraction to at least one freezing / thawing cycle such as to obtain a lysis of the platelets contained in the buffy-coat fraction; centrifuge said fraction and withdraw the liquid phase. The photochemical agent used is preferably a psoralen, the photochemical agent is used at a final concentration of 150 25 umol / L, UV rays are UVA and exposure to UVA rays is 3 J / cm2.

It is a product derived from healthy donors and which, free of contaminants with infectious potential, provides a standardized blend of growth factors for excellent cell growth and tissue regeneration. The final product represents a standardized source of stimulus for cell growth, free from microbiological contaminants and with highly reproducible activity. The product can be used to promote cell growth and the regeneration of tissues of mesodermal origin.

Possible Applications

• Cell growth stimulation for mesodermally derived human cells.

Advantages

• Absence of contamination with infectious potential;
• Valid substitute for Fetal Bovine Serum (FBS);
• Achievement of reproducible cell growth in the absence of xenogenic components;
• Obtaining a standardized blend of growth factors for optimal cell growth and tissue regeneration.