Overcoming the gold standard: Platinum labelling in bioimaging
This is a new bio-imaging method for immunocitochemistry, protein marking and nanoparticle cellular uptake experiments.
The new method, based on the intrinsic peroxidase-like activity of the platinum nanoparticles can be applied in many EM experiments where the well-known gold nanoparticles are used, including CLEM.
The platinum nanoparticles, as the gold ones, are easy to be functionalized with antibodies and/or fluorophores. But the platinum nanoparticles have a peculiarity: if used together with the diaminobenzindine (DAB) and the osmium tetroxide they are able to increase their electron dense signal intensity of at least 1 order of magnitude. Indeed oxidized DAB and osmium tetroxide form an electron-dense precipitate surrounding the nanoparticles themselves. This new method is interesting since it avoids costly, time consuming and artifact prone procedures that are nowadays employed in many EM experiments.
- Electron microscopy probes in nanoparticle cellular uptake experiments;
- Electron microscopy probes in protein marking experiments;
- Electron microscopy probes in pre-embedding immuno-gold experiments;
- CLEM bio-imaging probes.
- Easy to identify in electron microscopy at low magnification;
- Same electron-density, surface properties and chemistry as gold nanoparticles;
- Easy to be functionalized with antibodies and/or fluorophores;
- Facilitating multiple labelling allowing the simultaneous marking of two proteins;
- Suitable to study the intracellular fate of nanomaterials;
- Low cytotoxicity.