New method for cellular reprogramming
The authors describe the identification of the INT6-5a gene, a new isoform of the human INT6 transcript encoded by the EIF3E gene, and a related method that uses INT6-5a to increase the efficiency for generation of induced pluripotent stem cells (iPS) by the means of an epigenomic approach.
The generation of iPSCs using viral vectors is not recommended for clinical applications since the transgenes can lead to the activation or inactivation of oncogenes or tumor suppressor genes or can interrupt encoding regions essential for the cell. The technology here used for generating human iPS cells is compatible to clinical applications and do not involve genomic integration of reprogramming factors. The cellular reprogramming with the INT6-5a isoform was carried out using transient mRNA transfection methods for reprogramming of human fibroblasts in order to overcome the problems of safety related to the use of viral constructs. The transient transfection was performed with mRNA for INT6-5a, Oct4, Sox2, KFL4, low concentration L-Myc and Lin28.
- Generation of human iPS for clinical, biomedical and research applications;
- Generation of animal iPS for applications in veterinary and research practice.
- Increased efficiency of reprogramming intransigent cells to reprogramming;
- Reprogramming of terminally differentiated cells or hard to reprogram;
- Reprogramming of damaged or isolated cells from elderly patients by repealing their status.