Molecular test for antibiotic resistance

Introduction

Enterobacteria are non-spore-forming Gram-negative microorganisms whose natural habitat is the intestine, both human and animal. In recent years, enterobacteria have developed resistance to most beta-lactam antibiotics, these microorganisms known as ESBL (Extended Spectrum Beta Lactamase)producing microorganisms have become a serious public health problem due to their extreme diffusibility.

Technical features

The invention is a multiplex RT pcr for the detection of genes that confer resistance to beta-lactam antibiotics (bla tem, bla ctm, bla shv and bla imp, bla per, bla mir, bla fox, bla fim, bla dha, bla acc , bla cmy, bla veb, bla oxa23) resistance to colistin and an internal control to ascertain the presence of a microorganism belonging to the Enterobacteriaceae family. The test can be performed directly on bacterial colonies and rectal swabs, while on blood cultures and other biological fluids, after the extraction of nucleic acids, in about 60 minutes. The sensitivity of the reaction is 25 CFU. The test uses 5 fluorescent probes per reaction tube (4 for the targets plus one for the internal control) divided into 3 tubes.

Possible Applications

•   clinical-diagnostic

•   antimicrobial stewardship

Advantages

Rapid evaluation of beta lactam resistance

Rapid evaluation of colistin resistance

Rapid change of empirical therapy based on test result