Politecnico di Torino - Corso Duca degli Abruzzi, 24 - 10129 Torino, ITALY

+39 011 090 6100 info@tech-share.it

Microscope on chip for 3D imaging of cells

3D fluorescence microscopeFs-laser micromachiningHigh-throughput screeningLab-on-chipMicrofluidicsTechShareDay-d2w2

Introduction

The proposed optofluidic device allows performing light sheet fluorescence microscopy on a millimiter-scaled glass chip. The fluorescent samples are selectively excited while they sequentially flow in the channel, thus allowing the the 3D image reconstruction. Our device can be used as add-on in any wide-field microscope, it does not require alignment of the light sheet on the sample and allows high-throughput measurements.

Technical features

The selective plane illumination microscopy techniques allows reconstructing 3D images with high spatial resolution and minimal phototoxic damage of the samples. One of the limits of this technique is the throughput, infact it requires to image a sample per time by positioning it and aligning it with a specific holder. The integrated optofluidic device is realized by structuring a glass substrate through femtosecond laser irradiation followed by chemical etching. The technique allows obtaining selective plane illumination microscopy on a millimiter scaled lab on chip. An integrated cylindrical lens fabricated in the chip is used to generate a light sheet in a microfluidic channel used to deliver the samples. The optical sectioning that alloes selective fluorescence excitation is therefore achieved by flowing the sample through the ligh sheet. The continuous sample flow allows measuring many samples in a short time. The integrated device can be used as add-on in a standard microscope permitting fluorescence 3D microscopy in an automated manner, thus reducing the operator intervention.

Possible Applications

  • Morphological analyses of single cells or clusters;
  • Analysis of nuclear or sub-nuclear markers;
  • High throughput screening of biological samples;
  • Drugs efficacy monitoring.

Advantages

  • Morphological analyses of single cells or clusters;
  • Analysis of nuclear or sub-nuclear markers;
  • High throughput screening of biological samples;
  • Drugs efficacy monitoring.