Politecnico di Torino - Corso Duca degli Abruzzi, 24 - 10129 Torino, ITALY

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CosmeceuticalsIn vitro callogenesisNutraceuticalsProduction of secondary metabolitesTriterpenic acids


The invention refers to a method for the production of large quantities of different triterpenic acids through an in vitro culture of callus deriving from apple pulp of the Red Sentinel (RS) variety, and in vitro culture of callus deriving from the pulp of the RS apple obtainable by this method. Furthermore, the method of the present invention promotes a stable and continuous production of triterpenic acids for long periods of time.

Technical features

The present invention provides a method for the production of triterpenic acids from biological material grown in vitro, “callus”, deriving from RS apple pulp. The excellent technical effect observed, is the significant increase in the quantities of triterpenic acids produced starting from the callus compared to the yields obtained by using different vegetable matrices known in the state of the art. The average quantity of all triterpenic acids produced according to the method of this invention is significantly higher both: concerning the quantity obtained from callus deriving from the pulp of other apples; and concerning the quantity of triterpenic acids directly extracted from the skins or pulp of the RS apple. This increase is possible thanks to the in vitro culture of plant material deriving from RS pulp on a culture medium added with cytokinin/auxin growth-regulating compounds in a specific ratio. These culture conditions favor the optimal development of callus and the high content of triterpenic acids. The extracts obtained by the method of the invention include: oleanolic acid; ursolic acid; maslinic acid; corosolic acid; annurcoic acid; and tormentic acid.

Possible Applications

Nutraceutical; Cosmeceuticals; Pharmaceutical; Cosmetics.


Development of a cell culture, “callus”, starting from the pulp of the RS apple variety; Strong quantitative increase in the production of triterpenic acids; Overcoming the concept of «balsamic time»; Guarantee of constant and regular production over time of plant material and secondary metabolites.