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Method for in vitro diagnosis of allergies and related device

Allergy diagnostic testingIgE-allergen reaction kineticsImpedence measurements


A method for the detection and quantification of specific immunoglobulins E (IgE) for an allergen of interest in a patient’s serum sample, and a device for achieving this method.

Technical features

The interaction between the IgE and allergen occurs through a bonding mechanism. Following this interaction some of the free proteins/electrons of the immunoglobulin structure come closer to the allergen structure, thus enhancing or reducing the impedance value of the serum under testing. Consequently, the higher the difference in measured impedance values the higher the interaction between the IgE and the allergen under testing, the latter at an optimal concentration between 10 and 150 mg/mL. The measurement of impedance values in serum samples are carried out in alternate current regime at a frequency from 10 Hz up to 100kHz with a resolution of at least 1 Hz to minimize any biasing effects from interacting substances present in the serum. These substances may get polarized if biased with constant voltage, so minimisation of the bias ensures the preservation at highest grade of the serum properties.

Possible Applications

  • In vitro quantitative evaluation of allergic reaction;
  • Rapid estimation of allergic reaction gravity;
  • Alternative to traditional allergy diagnostic kits;
  • Safety increase for excessive allergy reactions measurements.


  • High accuracy;
  • Dynamic impedance measurement;
  • Reaction kinetics studies;
  • Simple, fast and low cost.