Marinomonas ef1 and Rhodococcus ef1 for the production of secondary metabolites
This invention is related to the field of microbiology and concerns the characterization of two novel bacterial species belonging to Marinomonas and Rhodococcus genera, and the use of these bacteria in methodologies for the “in vitro” production of usefull metabolites. In particular, the patent concerns the biosynthesis by using these bacteria of fluorescent molecules and of silver nanoparticles with antibiotic activity against several pathogenic microorganisms.
The present patent describes two novel bacterial strains isolated in our laboratories and deposited according to the Budapest treaty. These strains are able to produce secondary metabolites for useful applications. These bacteria are cold tolerant and can growth in a wide range of temperatures, from 4 °C to 28 °C. In the presence of glucose and peptone in the culture medium, these bacteria produce fluorescent molecules that can be exploited as natural dyes. Moreover, in the presence of silver nitrate, these strains are able of silver nanoparticles biosynthesis. These nanopaticles show high antimicrobial activity against pathogenic gram-positive and gram-negative bacteria and against pathogenic yeast such as Candida. The production of the silver nanoparticles is rapid and low-cost. Furthermore, the biosynthesis is environmentally friendly since does not imply the use of toxic substances for nanoparticles purification. In particular, we aim in the production of materials such as tissues or sponges that contain the bio-produced silver nanoparticles to be used to sterilize surfaces from pathogenic agents.
- In the bio-medical industry: production of materials containing antimicrobial nanoparticles for surfaces cleaning;
- In the footwear industry: production of antimicrobial soles;
- Production of pavements and paints with antimicrobial activity.
- Availability of bacteria that are able to produce secondary metabolites and can growth within a wide range of temperatures;
- Availability of materials with antimicrobial activity;
- Low environmental impact;