LIpHOSOME FOR DOSING METHODS
Over the past 50 years, numerous methods have been developed for the in vitro detection of diagnostic markers in biological fluids. These analyses often rely on antigen / antibody recognition (immunoassay). However, there is a great need to develop methods with higher sensitivity, lower costs and higher simplicity to be performed.
The present invention relates to a composition of liposomes containing an aqueous solution at a different pH with respect to the pH of the solution in which they are suspended. Such a liposome has at least one specific ligand on the surface for an analyte of interest contained in a biological fluid. This feature allows exploiting this system in dosing methods. The presence of the liposome, possibly linked to the ligand of interest, is highlighted through a detectable pH variation by means of a pH meter or, more banally, an indicators or litmus paper. This new method is called LICIA (LIpHosomes Conjugated Immunosorbent Assay). Compared to the used ELISA test, the LICIA test requires low-cost instrumentation available to any scientific laboratory (pH meter), and the necessary molecules have a lower cost. Furthermore, the pH meter requires fewer skills than those required for ELISA instrumentation.
- Immuno-enzymatic assays;
- In vitro diagnostic tests;
- Liquid biopsies;
- Dosing methods;
- Agri-food analysis;
- Environmental analysis.
- Reduction of preparation costs;
- Easy use by unskilled personnel;
- Use of low-cost equipment in any laboratory;
- Wide method versatility: The LipHosoma can be functionalized to detect different targets in various diagnostic kits.