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GRAPHENE BIOSENSOR

AntibodycancerCancroExosomesGrafeneGrapheneRecognizing deviceTumori

Introduction

The invention relates to a device able to recognize, purify, and analyze exosomes from biological fluids. It is composed by a surface-modified graphene that can be supported on different substrates, including SiC or glass. The surface is modified with antibodies or aptamers which allow to bind the target. Thanks to this modification, exosomes are recognized with a high degree of specificity.

Technical features

Here we present the first all-in-one device able to recognize, purify, and analyze exosomes from biological fluids, the exosome graphene value check (EGVC) test. This chip is composed by a surface-modified graphene that can be supported on different substrates, including but not limited to SiC or glass. The modification of the graphene surface can be performed by various protocols, among which: i) a two steps process where the surface is functionalized with molecules able to interact with it and showing a click chemical group, followed by a reaction with antibodies or aptamers, or ii) a one step process that use modified aptamers or antibodies able to directly interact with the surface. Thanks to this modification, exosomes are recognized with a high degree of specificity. This is a key-goal, because exosomes do not aspecifically interact with the chip. After/during the recognizing step, the device can be analyzed by optical or electrical methods such as microscopy or electrical conductivity measurements, respectively.

Possible Applications

  • Isolation of exosomes and sub-populations of them with high specificity;
  • Diagnostics and early diagnostics;
  • Utilization in point-of-care devices;
  • Biological fluids analysis.

Advantages

  • Simplified Analisys;
  • It can be fabricated with existing technologies;
  • Multiple analyses;
  • All-in-one chip for the recognition, purification, and analysis of exosomes.