Introduction

A fluorescent dye, belonging to a class of molecules taking part in this invention, allows intracellular detection of the Golgi complex and of the derived vesicles membranes. The post-Golgi vesicle trafficking can be monitored without altering cell viability and functionality and without requiring cell permealization. The probe emits in the range of 520-550 nm and can be combined with other fluorochromes.

Technical features

The dye here shown belongs to a class of molecules taking part in the present invention; it is easy to synthesize even for a large scale production. The molecule penetrates the cell without any need for its permealization and binds to the phosphate group of the inner layer of membrane phospholipids where, in the absence of water, the irradiated fluorophore emits green light (525 nm) with high quantum yield. The dye shows a high chemical stability in solvents which keeps after irradiation, overcoming the deterioration of the sample usually found with the biormarkers. These properties allow the use in small amount by an easy protocol affording definite and brilliant detection of the biological target through fluorescence microscopy and flow cytometry. The dye specifically targets the perinuclear zone of the cell normally occupied by the Golgy apparatus and the vesicles in proximity of the Golgi cisternae. The easy use, the specific bological marking and the high chemical stability render the dye appealing.

Possible Applications

• Research studies;
• Disease detection.

Advantages

• Simple synthetic process;
• High chemical stability;
• High detection rate;
• Stock solution stable over time
• No cell permealizzation;
• Simple marker use protocol;
• Possible use in multiparametric analyses.