Introduction

The new ELISA kit provides for the quantitative measurement of serum levels of bone alkaline phosphatase, by exploiting the intrinsic catalytic activity of the protein itself. This allows the use of only one antibody in the assembly of the kit with a consequent significant reduction in costs and time for evaluating the result. The invention comprises method and kit.

Technical features

The kit allows the quantitative measurement of serum levels of bone alkaline phosphatase, by exploiting the intrinsic catalytic activity of the protein itself. This allows the use of only one antibody in the assembly of the kit with a consequent significant reduction in costs and time for evaluating the result. With this method, a greater sensitivity was also obtained than that of the diagnostic kits currently on the market. The final protocol involves two simple steps with the use of a single antibody. The kit was assembled in such a way as to allow its expansion and transformation from a kit, for the evaluation of the single marker, into a kit for the possible simultaneous evaluation of three markers. The peculiarity of this new ELISA diagnostic kit is also that of the potential expansion of the number of markers evaluated in the same assay due to the possibility of inserting other bone markers to be dosed (e.g. osteocalcin). The kit has been developed and finished. Experimental data were obtained using the kit on sera to assess sensitivity.

Possible Applications

• Skeletal tissue disorders for a differential diagnosis of skeletal diseases and other osteopenizing or ostensensating metabolic diseases of the skeleton;
• Monitoring of drug therapies;
• Field of clinical and basic research.

Advantages

• Greater speed of obtaining results;
• Reduction of total dosage costs;
• Widely usable and usable diagnostic method.