Politecnico di Torino - Corso Duca degli Abruzzi, 24 - 10129 Torino, ITALY

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ANALYTICAL METHOD FOR THE IDENTIFICATION OF

Alcohol abuse markercarbohydrate-deficient transferrinchromatographyFluorescence detectionLiver condition

Introduction

The invention represents an important contribution in both clinical and forensic diagnosis of alcohol abuse and hepatic metabolic liver disease. Thanks to this new methodology, there will be the advantage of using a specific marker to verify if a subject has an alcohol addiction problem. This latter affects negatively the health of the person or it can cause social problems (i.e. work accident or road accident).

Technical features

The invention is related to  an analytical method for the identification of at least one molecule of transferrin, an important iron-carrying protein. In particular the goal is to detect the carbohydrate-deficient transferrin (CDT) which is the most suitable marker for the evaluation of chronic abuse of alcohol. The detection and measurement of those molecules are carry out through a fluorescent detection system. The advantage of this methodology is the simplicity of application and the flexibility; indeed, it can be adapted to different types of tissues of body fluids from which the sample is taken. Furthermore, this method makes possible to draw graph where any noise due to the characteristic of the sample tissue, body fluids are not relevant compared to other methods. This allow to decrease the number of false positive. Furthermore, highly accurate results are obtained event if the sample is small. The most important advantage is that for a positive result there is no need to carry out further test as required with the current methods.

Possible Applications

  • Medical diagnostic; this test can be used in the diagnostic medical field to detect any alcohol addiction and even the degree of severity of the same. Furthermore, it can be used to identify any metabolic illness of the liver;
  • Forensic science; the test can also be applied to support technical consultants in determining the causes of a specific biological lesion.

Advantages

  • Quick result test;
  • High accurancy;
  • Flexibility on test sample;
  • Diagnosis of metabolic liver disorder;
  • Easy methology.